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KMID : 0881720160310020094
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Journal of Food Hygiene and Safety
2016 Volume.31 No. 2 p.94 ~ p.98
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Simultaneous Analysis for Veterinary Drug Residues in Honey by HPLC/MS/MS
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Kim Jong-Hwa
Moon Sun-Ae
Kim Ki-Yu
Jung You-Jung
Lee Chang-Hee
Ku Eun-Jung
Yoon Mi-Hye
Lee Jong-Bok
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Abstract
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This study was conducted to establish the simultaneous analysis method for veterinary drug residues in honey by high performance liquid chromatography tandem mass spectrometry (HPLC/MS/MS). The eleven targeting veterinary drugs with honey test method in Korean Food Standards Codex were divided into Group 1 (streptomycine dihydrostreptomycine, neomycine) and Group 2 (oxytetracycline, enrofloxacin, ciprofloxacin, cymiazole, chloramphenicol, amitraz, coumaphos, fluvalinate) to be analyzed simultaneously. From the results, the retention time (RT) of the targeting drugs was within 15 min, the range of detection limits was 0.0056 to 0.0643 μg/g and the range of quantification limits was 0.0169 to 0.1948 μg/g. The coefficients of determination (R2) for Group 1 (0.05~1.0 μg/mL) and Group 2 (0.01~1.0 μg/mL) were 0.9917~0.9987 and 0.9923~1.000 respectively, and showed the good linearity. The recovery rates for Group 1 (final conc. 0.25 μg/g) and Group 2 (final conc. 1.0 μg/g) were 65.1~80.6% and 64.2~90.3% respectively. Also, the analysis results of inter day (n = 3) and intra day (n = 6) RSD (%) for area and retention time showed that the RSD (%) for area and retention time was below 10.92% and 1.57%. Therefore, the simultaneous analysis method of this study is evaluated to be a good test method for veterinary drug residues in honey.
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KEYWORD
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HPLC-MS/MS, honey, veterinary drugs, simultaneous analysis
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